Product Description:
Quantitive diagnosis of Hepatitis B virus in blood serum or blood plasma using TagMan probes.
Features:
-High sensitivity: 20 IU/mL dectection limit
-Internal control
-Cross-contamination control: Uracil-DNA glycosylase + dUTP
-Can be used for samples with high blood fat, high hemolysis, or drug treated samples
-Wide range of genotypes coverage: HBV A, B, C, D, E, F, G, H
Workflow:
Compatible DNA Extraction Kit:
Hybribio DNA Prep Kit (Cat. No HBDP)
Sample type: blood serum or blood plasma
Instrument requirements: FAM, HEX/JOE, ROX/Red 610, Cy5 fluorescent detection channels (LineGene 9660, ABI 7500, Rotor-Gene 3000, Mx3005P, LightCycler 480)
Standard Materials: Internal control, negative control, strong positive (1×106 IU/ml) and critical positive (100 IU/ml).
Specification:
Tests | 48 |
Sample Type | blood serum or blood plasma |
Device/instrument required | 4 channel Real-time PCR machine (Probes: FAM, HEX/JOE, ROX/ Red 610, Cy5) |
Sample volume Required | 1 ml |
Controls | Internal Controls (IC), External Controls |
Maximum run at a time | 1 test/reaction |
Result | Quantitative detection HBV infections |
Benefits:
– High throughput with short reaction time
– Easy to operate
– No specific RT-PCR instrument required, open platform
– Reduce unnecessary labor cost and reaction time